Generation of the chemiluminescence is an active process, which happens when the AquaSpark™ molecules are cut by the enzyme produced. Due to that, the signal builds for 5 to 15 minutes. After that time, fewer AquaSpark™ molecules are available in the solution and thus less light is generated. As a result, the signal starts decaying. To obtain the most reproducible results, ensure you measure chemiluminescent 5 minutes after the release of the AquaSpark™ tablet as detailed in our IFU.
