Incubating at higher or lower temperatures than 37°C will significantly affect the performance of the test and increase the risk of false negatives. Higher temperatures could negatively affect Listeria monocytogenes survival rate and growth properties and, lower temperatures slow down growth rate. Further, it has been demonstrated that Listeria monocytogenes can optimally express the trigger factor (PI-PLC) at 37°C and is significantly reduced at temperatures below 37°C. NEMIS can only guarantee reliable test performance at incubating at a constant temperature of 37°C.
