Results can be obtained after 24 hours of incubation at 37°C. A reduction of the incubation time by one hour (23 h) is also ok, but incubation times shorter than that will decrease the sensitivity of the N-Light™ Listeria spp. test. Likewise, up to six hours longer incubation time (30 h) will not affect the test, but longer incubation times have not yet been validated and may lead to unreliable results.
Developed N-Light™ Listeria spp. test tubes, safely closed with the biosafety cap, can be sent to a biosafety level 2 lab for follow-up analysis. NEMIS offers a tube cutter for opening of the biosafety cap. Presence of Listeria spp. and/or Listeria monocytogenes in the broth can be analyzed by molecular methods (PCR, real-time PCR, immunoassays) or by cultural methods such as ISO 11290-1.
