The test combines two core technologies: NEMIS bacteriophages (selective enrichment) and AquaSpark™, an ultrasensitive chemiluminescent molecule that reacts with the alpha-D-galactosidase enzyme produced by almost all Salmonella spp. and some other Enterobacteriaceae. In practice, the user follows a simple protocol:
- Collect the sample using a swab.
- Transfer it to the enrichment tube.
- Break and leave the swab inside the tube.
- Add the antibiotic tablet.
- Incubate it for 24 hours.
- Release the AquaSpark™ tablet into the tube.
- Incubate the activated tube at 37 ˚C for 3-4 minutes
- Measure results
If there is a risk of having Salmonella spp. that have grown in the selective broth, bacteria will initiate a reaction that generates light which the Luminometer measures, thus yielding a presumptive positive result.
