The test combines two core technologies: NEMIS bacteriophages (selective enrichment) and AquaSpark™, an ultrasensitive chemiluminescent molecule that reacts with an enzyme (PI-PLC) produced specifically by Listeria monocytogenes. In practice, the user follows a simple protocol:
- Collect the sample using a swab.
- Transfer it to the enrichment tube.
- Incubate it for 24 hours.
- Release the AquaSpark™ tablet into the tube.
- Incubate the activated tube at 37 ˚C for 5 minutes
- Measure results
If Listeria monocytogenes is present in your sample, they will initiate a reaction that generates light which the Luminometer measures, thus yielding a presumptive positive result. Figure 1 shows what happens when the AquaSpark™ tablet is released into a sample containing L.monocytogenes.
